| Title | Expression of CXCR4, the receptor for stromal cell-derived factor-1 on fetal and adult human lympho-hematopoietic progenitors. |
| Publication Type | Journal Article |
| Year of Publication | 1999 |
| Authors | Aiuti A, Tavian M, Cipponi A, Ficara F, Zappone E, Hoxie J, Peault B, Bordignon C |
| Journal | European journal of immunology |
| Volume | 29 |
| Issue | 6 |
| Pagination | 1823-31 |
| Date Published | 1999 Jun |
| ISSN | 0014-2980 |
| Keywords | Adult, Antigens, CD34, B-Lymphocytes, Chemokine CXCL12, Chemokines, CXC, Cytokines, Down-Regulation, Fetus, Hematopoiesis, Hematopoietic Stem Cells, Humans, Liver, Receptors, CXCR4, Stromal Cells, T-Lymphocytes |
| Abstract | Stromal cell-derived factor-1 (SDF-1) is a CXC chemokine produced by stromal cells that acts as a chemoattractant for human CD34+ progenitor cells. We investigated the expression of CXCR4, the receptor for SDF-1, on CD34+ cells from different hematopoietic sites and developmental stages. CXCR4 was detected by flow cytometry on 37 % of fetal bone marrow (BM) [gestation weeks (gw) 14-23] and 40% of adult BM CD34+ cells. Interestingly, in fetal liver CD34+ cells, CXCR4 was expressed at lower levels at later stages (9%, gw 20-23) compared to early stages of development (39%, gw 7.5-18), suggesting a development-related change in the migratory capacity of progenitors. CXCR4 was detected at similar levels on both phenotypically primitive and committed progenitors from fetal and adult sites. However, B cell lineage progenitor and precursor cells expressed CXCR4 at the highest density (80% of BM CD34+/CD10+ pro-B cells are CXCR4+). CXCR4 was also expressed in the fetal thymus in early T cell precursors and found to be down-regulated during T cell maturation. Finally, we found that stem cell factor, alone or in combination with other cytokines, can up-modulate CXCR4 expression on CD34+ cells by three- to fourfold. In conclusion, our results suggest that CXCR4 may play an important role in the local and systemic trafficking of human CD34+ cells as well as in human B lymphopoiesis and that its expression can be modulated by cytokines. |
| Alternate Journal | Eur. J. Immunol. |
